FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9617943
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC524990.5458443660978236TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG63200.063.1318446
GTATGCC70100.057.26692245
CGTATGC71700.055.8913744
ATGCCGT72050.055.47471647
TGCCGTC75450.052.9762448
TCGTATG78950.050.8031843
GCCGTCT81450.049.3315849
CTCGTAT81650.049.03698342
AAAAGGG90250.043.8986270
CCCGTCC94100.042.02663833
TCTCGTA94800.041.93934641
CCGTCTT103000.039.31609350
ACCCGTC103300.038.4873832
CATCTCG104950.037.5499939
AAAAAGG108550.036.4662669
ATCTCGT110500.035.9807340
TGAAAAA111950.035.9274762
CTGCTTG114400.035.43158757
CCGTCCA112400.035.27781734
CACCCGT113750.035.10527431