Basic Statistics
Measure | Value |
---|---|
Filename | HL7MJBGXY_n01_chl16.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9617943 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC | 52499 | 0.5458443660978236 | TruSeq Adapter, Index 16 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 6320 | 0.0 | 63.13184 | 46 |
GTATGCC | 7010 | 0.0 | 57.266922 | 45 |
CGTATGC | 7170 | 0.0 | 55.89137 | 44 |
ATGCCGT | 7205 | 0.0 | 55.474716 | 47 |
TGCCGTC | 7545 | 0.0 | 52.97624 | 48 |
TCGTATG | 7895 | 0.0 | 50.80318 | 43 |
GCCGTCT | 8145 | 0.0 | 49.33158 | 49 |
CTCGTAT | 8165 | 0.0 | 49.036983 | 42 |
AAAAGGG | 9025 | 0.0 | 43.89862 | 70 |
CCCGTCC | 9410 | 0.0 | 42.026638 | 33 |
TCTCGTA | 9480 | 0.0 | 41.939346 | 41 |
CCGTCTT | 10300 | 0.0 | 39.316093 | 50 |
ACCCGTC | 10330 | 0.0 | 38.48738 | 32 |
CATCTCG | 10495 | 0.0 | 37.54999 | 39 |
AAAAAGG | 10855 | 0.0 | 36.46626 | 69 |
ATCTCGT | 11050 | 0.0 | 35.98073 | 40 |
TGAAAAA | 11195 | 0.0 | 35.92747 | 62 |
CTGCTTG | 11440 | 0.0 | 35.431587 | 57 |
CCGTCCA | 11240 | 0.0 | 35.277817 | 34 |
CACCCGT | 11375 | 0.0 | 35.105274 | 31 |