FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9621337
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC1896061.9706824529688545TruSeq Adapter, Index 15 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127800.13282977199530585No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG213750.066.1979546
GTATGCC218950.064.68970545
CGTATGC223050.063.516344
ATGCCGT223850.063.29029547
TGCCGTC225250.062.8823748
TCGTATG228650.061.9453843
GCCGTCT228650.061.88672649
CTCGTAT231750.060.4670842
AAAAGGG235250.059.71716370
TCTCGTA245900.056.9451841
CCGTCTT252250.056.23518850
AAAAAGG255850.055.00559669
TGAAAAA263400.053.99598762
AATCTCG259650.053.90263439
ATCTCGT261300.053.5890540
CTGCTTG264800.053.57497457
GTCAATC262150.053.38803536
GAAAAAA280050.050.81099363
ACATGTC282450.050.3437332
TTGAAAA284500.050.0026361
Produced by FastQC (version 0.11.2)