FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11667733
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC1585261.3586701032668471TruSeq Adapter, Index 14 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG172350.065.6541646
GTATGCC179700.063.02723345
CGTATGC182650.061.9507344
ATGCCGT184000.061.5173347
TGCCGTC188450.060.06519348
TCGTATG188700.059.94569443
GCCGTCT193150.058.73095349
CTCGTAT193800.058.11359842
AAAAGGG204650.054.98291470
TCTCGTA211150.053.42178741
CCGTCTT223300.050.97416350
CATCTCG220700.050.85664439
AAAAAGG226700.049.74349269
TGAAAAA233600.048.76136462
ATCTCGT231000.048.7405240
CTGCTTG235100.048.24035357
ACAGTTC240050.047.49993532
TGCTTGA254950.044.58054458
GAAAAAA258150.044.21927663
TTGAAAA260200.043.95067261
Produced by FastQC (version 0.11.2)