FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13503836
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC1514781.1217405187681484TruSeq Adapter, Index 13 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG178100.064.770146
GTATGCC184800.062.30843745
ATGCCGT189800.060.85141447
CGTATGC192600.059.89407344
TGCCGTC194300.059.51546548
TCGTATG198150.058.12775443
GCCGTCT200600.057.6467649
CTCGTAT205700.055.53443542
AAAAGGG212750.053.6618670
TCTCGTA227550.050.21761341
CCGTCTT233950.049.45994650
TGAAAAA242900.047.61276262
AAAAAGG241650.047.24489669
CTGCTTG251550.046.2246157
AATCTCG254100.045.0259839
ATCTCGT255750.044.79023440
GAAAAAA271550.042.75708463
GTCAAAT270700.042.66504335
TTGAAAA273250.042.40124561
TGCTTGA280600.041.55163658
Produced by FastQC (version 0.11.2)