FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6612413
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10400.048.12442446
CGTATGC15400.032.95390344
GTATGCC15400.032.72688345
ATGCCGT18300.027.15793847
GGCGCGC4150.026.14307838
TGCCGTC19150.026.13545648
TCGTATG21450.023.65903343
GCCGTCT23150.022.52689749
CTCGTAT22850.022.0561342
GCGCGCA5600.020.62381739
CGCGCAC5700.018.42041440
CGCGACT4950.018.3911291
CGGGGGT2901.8189894E-1118.1106281
CCCCCCT1551.9801573E-518.0716781
AAAAGGG30000.016.44955470
CCCGTGT6800.015.962211
GGCTACA32100.015.59088734
TCTCGTA32850.015.44847541
CGGCTAC32950.015.29479533
CTACATC37250.014.09315136
Produced by FastQC (version 0.11.2)