FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6969686
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10300.044.17343546
GTATGCC14350.032.43828645
CGTATGC15150.030.26312444
ATGCCGT18050.025.40126847
GGCGCGC3400.023.67525138
TGCCGTC20250.022.81494948
TCGTATG20500.022.70582443
CTCGTAT21200.021.9562642
GCCGTCT21300.021.52610449
TATCTCG24000.019.68662839
CGCGCAC4100.019.63327640
AAAAGGG30700.016.07478370
GCGCGCA5150.014.95079339
TCTCGTA32950.014.44549141
GCGGACT3202.2746462E-814.2240391
CGCCAAT17450.013.2427461
CCCGGGG4558.367351E-1113.0817881
GCTTATC37050.013.03567436
CGGGGTG1950.00229587612.5687751
TCGGGGG7550.012.5174392
Produced by FastQC (version 0.11.2)