FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6924265
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10850.036.45072646
GTATGCC14450.027.61238345
GGCGCGC4200.024.9986738
CGTATGC16250.024.98454744
TGCCGTC17950.022.423548
ATGCCGT18000.021.97184647
CGGGGCG850.003783051220.5972541
CGCGCAC5450.019.90791740
GCCGTCT21800.019.26628349
TCGTATG22100.018.68773343
GCGCGCA5900.017.79604739
CTCGTAT23300.017.42459542
CGGGCCC1400.003745530515.0065711
CGGGGGT3302.1300366E-914.854991
CGCGTAT5750.014.6150941
AAAAGGG29150.014.40777870
GCGCACC8150.013.74209741
TCGGGGG5950.013.5293752
GGCGCCC7250.013.51827967
GTCCGCG6800.012.86947260
Produced by FastQC (version 0.11.2)