FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8511547
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87250.1025078049853922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13100.042.4800346
GTATGCC19000.029.65727845
ATGCCGT20150.028.31206547
CGTATGC19850.027.8577144
GGCGCGC4750.023.57790238
TGCCGTC24050.023.2847548
TCGTATG26400.021.47619443
CTCGTAT27000.020.869242
GCCGTCT28150.020.26660349
GCGCGCA6400.018.59292639
CGCGCAC6550.016.56424740
CCCCGGG8000.014.87670162
AAAAGGG38250.014.45704670
CGCGGGT7400.014.193731
CATGCGC8900.013.76521465
TCTCGTA43650.013.39001441
GCGCTAA8250.013.15147468
GGGCCGC3601.0681106E-712.63996354
GGGGTAT10300.012.5732923
TGCGCTA9550.012.4612867
Produced by FastQC (version 0.11.2)