FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7624270
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC110680.14516799641145972TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16550.048.00531846
GTATGCC20900.038.1814945
CGTATGC23050.034.61963744
ATGCCGT23300.034.24818447
TGCCGTC26300.030.34214248
GCCGTCT28450.028.54161549
TCGTATG28900.027.73277943
CTCGTAT31000.025.85393742
GGCGCGC4200.023.33267238
AAAAGGG37350.021.64611670
TCTCGTA42950.018.57909641
TAGGCAT45650.017.7101135
CCGTCTT47800.017.64710250
TTAGGCA48500.016.88589934
ACTTAGG47700.016.72864732
CATCTCG47700.016.58223239
CGCGCAC6250.016.23964740
AAAAAGG52700.015.73990269
GGCATCT50800.015.57042637
GCGCGCA6350.015.43253339
Produced by FastQC (version 0.11.2)