FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7728687
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC798261.0328533164818294TruSeq Adapter, Index 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110220.1426115457903781No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG91500.063.3426446
GTATGCC95300.060.81730345
ATGCCGT96800.059.7664247
CGTATGC97900.059.20213744
TGCCGTC99950.058.09406348
GCCGTCT102200.056.67883349
TCGTATG104550.055.40269543
CTCGTAT106850.054.1115142
AAAAGGG110400.052.05516470
CCGTCTT118550.049.15775750
TCTCGTA118850.048.5305241
AAAAAGG124500.046.32872869
TGAAAAA129700.045.17904362
CTGCTTG131800.044.2712857
ATCTCGT132300.043.62350540
GATCTCG139550.041.331839
TTGAAAA142500.041.2426161
CACGATC139700.041.0864636
TCACGAT141050.041.0664835
ACGATCT140500.040.97733337
Produced by FastQC (version 0.11.2)