FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4913387
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC73540.14967272067109716TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10400.048.46092646
GTATGCC14700.034.76181845
CGTATGC15300.033.16985744
TCGTATG17050.030.17587743
ATGCCGT16750.030.08917247
TGCCGTC17250.029.82540748
GCCGTCT19300.026.47634949
CTCGTAT19500.026.02504542
AAAAGGG27050.018.76112470
TCTCGTA27850.017.97086541
CCGTCTT30750.017.52876950
GGCGCGC2452.4833935E-715.71392738
TGAAAAA35100.014.95905562
AAAAAGG36300.014.17322969
CTGCTTG38650.014.12789357
AATCTCG37200.013.92441439
ATCTCGT36900.013.84792140
TGTAATC38400.013.39785936
GTAATCT39350.013.07453737
TTGTAAT43300.013.01342835
Produced by FastQC (version 0.11.2)