FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6882787
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7950.039.6212746
GGCGCGC4050.025.06085838
ATGCCGT14050.023.16645647
GTATGCC14100.022.58819845
CGTATGC15600.020.86481344
CGCGCAC5400.018.14778140
GCGCGCA5950.017.64657639
TGCCGTC18800.017.31373248
TCGTATG19150.016.99692543
GCCGTCT21450.015.33802149
CTCGTAT21350.014.91751542
GCGCTAA5750.014.0004368
GCGTGCG6250.012.87917919
CGCGTGT4255.5624696E-912.3567331
GCGCACC8450.012.01141841
GGGGCCC2952.8968458E-511.86408143
ACATGCG6850.011.75312564
CGCGCGT2408.1577647E-411.6702461
TGCGCTA7200.011.6672867
AAAAGGG29150.011.52627170
Produced by FastQC (version 0.11.2)