FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7917875
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC122060.15415752332538718TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18250.049.09397546
GTATGCC22650.039.86624545
CGTATGC23550.038.04498744
ATGCCGT23750.037.72532347
TGCCGTC29000.031.37868348
TCGTATG29600.030.1504743
GCCGTCT32550.027.84890649
CTCGTAT33800.026.40396342
TATCTCG35300.025.77754439
GGCGCGC4650.021.82655538
AAAAGGG40950.021.70841870
TCTCGTA45150.020.77412241
GTCACGC50350.017.86413629
CACGCCA50700.017.80984531
CCGTCTT53250.017.41782250
ACGCCAA52650.017.34964232
TCACGCC52200.017.2310230
CGCCAAT52800.017.1013933
AAAAAGG57250.016.07787569
TGAAAAA58900.016.04645562
Produced by FastQC (version 0.11.2)