FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7604151
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC77230.10156294897352774TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10950.044.10800646
GTATGCC16700.030.38853345
CGTATGC17050.029.14851444
ATGCCGT19550.025.06320447
GGCGCGC4000.024.49863638
TCGTATG20850.024.17147643
TGCCGTC21050.023.44395648
GCCGTCT21500.023.27915449
CTCGTAT23900.020.94025842
CGCGCAC5400.018.79537440
GCGCGCA6000.018.6657539
CGCCCCT4400.015.914381
CGGGGGT2755.5000783E-815.2778051
GCGCTAA6700.015.149507568
AAAAGGG33950.014.94762370
TGCGCTA6800.014.9273167
CGCGTAT5400.013.6156361
GGGGCCC2254.756236E-412.44383443
CCGTCTT42300.012.32889350
TCTCGTA39900.012.2801841
Produced by FastQC (version 0.11.2)