FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8749228
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC215560.24637602311883977TruSeq Adapter, Index 4 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89720.10254619036102386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27750.053.60279546
GTATGCC33400.044.9541645
CGTATGC33850.044.45993444
ATGCCGT36100.041.3013247
TCGTATG39950.037.6708843
TGCCGTC40650.036.93692848
GCCGTCT43900.033.96362749
CTCGTAT43750.033.75833542
AAAAGGG54150.027.72732270
TCTCGTA57400.025.91335741
CCGTCTT64950.023.54947350
ACTGACC65850.022.85358432
CACTGAC67800.022.55762531
AAAAAGG70400.021.5265469
TGAAAAA71750.021.36846462
ATCTCGT70800.021.05828540
AATCTCG71550.020.78851339
GGCGCGC4650.020.32146338
CTGCTTG78450.019.89954657
GACCAAT79950.018.86689835
Produced by FastQC (version 0.11.2)