FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10424910
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC460440.44167287775146263TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG55850.059.34468546
GTATGCC62950.052.7071745
CGTATGC63800.052.1692844
ATGCCGT64300.051.8180447
TCGTATG69650.047.83753243
TGCCGTC70100.047.4818848
GCCGTCT72000.046.3261149
CTCGTAT74600.044.38183242
TATCTCG77500.042.76686539
AAAAGGG84950.038.9340670
CCGTCTT97250.034.55025550
TCTCGTA96800.034.3118241
GATGTAT101650.032.91572635
GTATCTC103900.032.30428738
CCGATGT103550.032.04091633
ATGTATC105350.031.49392136
AAAAAGG108950.030.5188469
TGTATCT109700.030.46869337
CGATGTA109700.030.40459334
TGAAAAA112250.030.15415862
Produced by FastQC (version 0.11.2)