FastQCFastQC Report
Mon 9 Oct 2023
HL557DRX3_n01_EMS18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL557DRX3_n01_EMS18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9481526
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGTAT1286481.3568280042685112TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGAT616820.6505492892177904TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGTTT358280.37787166327445604TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGTT280850.29620759358778326TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGGT95700.10093311983746076TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)