FastQCFastQC Report
Mon 9 Oct 2023
HL557DRX3_n01_EMS03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL557DRX3_n01_EMS03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41541874
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGTAT625208715.050084163271016TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCGCGTAT1870090.45016986956341926TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGGAT807540.19439180813075502TruSeq Adapter, Index 22 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGTA473300.11393323276653335TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)