FastQCFastQC Report
Mon 9 Oct 2023
HL557DRX3_n01_Ctrl04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL557DRX3_n01_Ctrl04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999392
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATCTCGTAT37930237.953275591559674TruSeq Adapter, Index 1 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATCGCGTAT103931.0399322788255259TruSeq Adapter, Index 1 (97% over 39bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATCTCGTA35490.35511591047356794TruSeq Adapter, Index 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)