FastQCFastQC Report
Mon 9 Oct 2023
HL557DRX3_n01_Cisp13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL557DRX3_n01_Cisp13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4262441
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATCTCGTAT176523841.41378144588981TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATCGCGTAT831031.9496574849951TruSeq Adapter, Index 20 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATCTCGTA107560.25234366880386144TruSeq Adapter, Index 18 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATCTCGTATG60220.1412805479301649TruSeq Adapter, Index 20 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)