FastQCFastQC Report
Mon 9 Oct 2023
HL557DRX3_n01_Cisp10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL557DRX3_n01_Cisp10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9556726
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGTT878870.9196350298208821TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGGT538660.5636449135404741TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGTTT461030.48241416568812373TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGAT458610.4798819177195202TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGTAT450710.4716154884005255TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGTGT207120.2167269418417981TruSeq Adapter, Index 16 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)