FastQCFastQC Report
Mon 9 Oct 2023
HL557DRX3_n01_Cisp02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL557DRX3_n01_Cisp02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8784279
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTACCAATCTCGTAT5656706.439572331434373TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTACCAATCGCGTAT164100.18681100634440231TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTACCAATCTCGGAT88220.10042941486717351TruSeq Adapter, Index 15 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)