FastQCFastQC Report
Tue 3 Oct 2023
HL553DRX3_n02_S4_D28_452_BCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL553DRX3_n02_S4_D28_452_BCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58663034
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4758510.8111598864797889Clontech SMART CDS Primer II A (100% over 26bp)
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT2375320.4049091630685177No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA922560.15726428333045303No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT921610.15710234148475852No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT862570.14703808193759635No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT832110.14184571496932805No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA751740.12814543482357219No Hit
GTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGT683140.11645152891342102No Hit
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCC643690.10972668068958043No Hit
CAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTT590410.10064430012262919No Hit
CCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAG586650.10000335134388036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)