FastQCFastQC Report
Tue 3 Oct 2023
HL553DRX3_n02_S3_D7_452_GEX.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL553DRX3_n02_S3_D7_452_GEX.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221634888
Sequences flagged as poor quality0
Sequence length90
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2488092711.226087744813894Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18323830.8267574733089856Clontech SMART CDS Primer II A (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17318550.7813999933079128Clontech SMART CDS Primer II A (100% over 24bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4217010.19026832995715007Clontech SMART CDS Primer II A (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG3244670.14639707806290858Clontech SMART CDS Primer II A (100% over 26bp)
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA2454850.11076099174422395No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA2319980.10467575844828184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)