FastQCFastQC Report
Tue 3 Oct 2023
HL553DRX3_n02_S1_D0_452_BCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL553DRX3_n02_S1_D0_452_BCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86817979
Sequences flagged as poor quality0
Sequence length90
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36661144.2227589748432175No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA15430221.7773069792375609No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT7416620.8542723621797277No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT4345860.500571431177867No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT3814990.4394239584867554No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT1910910.220105330947637No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1910800.2200926607609698Clontech SMART CDS Primer II A (100% over 26bp)
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC1480960.1705821786061157No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG1364440.15716099542008458No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG1297000.1493930191579327No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC1182570.13621256951857863No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT1146590.13206826664324908No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC1050950.12105211525368495No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA883780.10179688702497902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)