FastQCFastQC Report
Tue 3 Oct 2023
HL553DRX3_n01_S4_D28_452_TCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL553DRX3_n01_S4_D28_452_TCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67021552
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATATAAATGCGTAGGGGTTTT4994880.745264747077179No Hit
ATTGGGCTGTTAATTGTCAGTTCAGT1928610.2877596746789749No Hit
TGCTGTGTTATGCCCGCCTCTTCACG1852860.27645734017021867No Hit
AGTTGGTTGATTGTAGATATTGGGCT1768050.2638032016924944No Hit
AGGGGTTTTAGTTAAATGTCCTTTGA1763710.2631556487978673No Hit
GTGTTGGGTTGACAGTGAGGGTAATA1227850.18320226305711332No Hit
GGGCAGGCGGTGCCTCTAATACTGGT1103180.16460078393887387No Hit
ATGAGGGTAATAATGACTTGTTGGTT1099960.16412034146866666No Hit
TTTGGTTGATTGTAGATATTGGGCTG1014460.15136325103304082No Hit
GCACCTGCGGCGGCTCCACCCGGGCC946140.1411695151434273No Hit
GGGGTAGAAAATGTAGCCCATTTCTT802130.11968239708922289No Hit
TGGTTGATTGTAGATATTGGGCTGTT780570.1164655214191399No Hit
TGCTACCTTTGCACGGTTAGGGTACC733580.10945434388030884No Hit
ACTTGTTGGTTGATTGTAGATATTGG728400.10868145816736682No Hit
GACGCGGGCTTATGCGGAGGAGAATG690480.10302357665486468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)