FastQCFastQC Report
Tue 3 Oct 2023
HL553DRX3_n01_S4_D28_452_BCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL553DRX3_n01_S4_D28_452_BCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58663034
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATATAAATGCGTAGGGGTTTT4386400.7477281178467516No Hit
AGTTGGTTGATTGTAGATATTGGGCT1602980.27325214716988555No Hit
ATTGGGCTGTTAATTGTCAGTTCAGT1547850.26385440616658185No Hit
AGGGGTTTTAGTTAAATGTCCTTTGA1528820.26061045529966964No Hit
TGCTGTGTTATGCCCGCCTCTTCACG1436240.2448287962739875No Hit
GTGTTGGGTTGACAGTGAGGGTAATA947370.1614935224795908No Hit
TTTGGTTGATTGTAGATATTGGGCTG904530.1541907975642719No Hit
ATGAGGGTAATAATGACTTGTTGGTT874000.14898649803895245No Hit
GCACCTGCGGCGGCTCCACCCGGGCC867870.147941546971471No Hit
GGGCAGGCGGTGCCTCTAATACTGGT759940.12954324864956696No Hit
AGGGTGATAGATTGGTCCAATTGGGT734230.12516059090977122No Hit
GACGCGGGCTTATGCGGAGGAGAATG723320.12330081666079527No Hit
GGGGTAGAAAATGTAGCCCATTTCTT700920.11948239840441938No Hit
TGGTTGATTGTAGATATTGGGCTGTT679370.11580887548366489No Hit
GGGCTGTTAATTGTCAGTTCAGTGTT637960.10874991566239142No Hit
TGGGCTGTTAATTGTCAGTTCAGTGT602040.10262680924413149No Hit
ATTGGGTGTGAGGAGTTCAGTTATAT600300.10233019996885943No Hit
ACGGGCTGTTAATTGTCAGTTCAGTG594780.10138923261282394No Hit
TGCTACCTTTGCACGGTTAGGGTACC593750.10121365355907094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)