FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24601643
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3275161.331276939511723No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN756320.30742662187236847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACTG24550.012.9866146
GTTCGGC7200.012.62034914
GAGCGCT3802.6512098E-611.1042393
GTAGGCT12300.010.82091641
CGCCGTA12050.010.76406247
TAAACTA59050.010.6203596
ACATGGG9850.010.31848824
TAGGCTA13100.010.161098542
CGGTTCG10150.09.98795412
CACTCTT20850.09.9594882
AGGCTAT13400.09.93767643
CCGTATC12100.09.84902949
CGCAGAG8900.09.83163816
GCCGTAT15450.09.755362548
AGCGGTT13750.09.66504110
CTAAACT26150.09.5315845
GTAAACT23500.09.1125595
CGTATCA13750.08.91207150
TCGCCGT16750.08.58023746
GGCTATA15350.08.44812244
Produced by FastQC (version 0.11.5)