FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_ColTGA5_Col_UB_i3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_ColTGA5_Col_UB_i3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22403
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6652.9683524527965006No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC1750.7811453823148686Illumina Single End PCR Primer 1 (96% over 33bp)
CATTGTAATATAAGGTTTGTACTTTCGATGTGAGATTTTTAACACAATTA280.12498326117037896No Hit
CATGTTCAGTGGCCATAACCTGACACGTGTACGGTGGAAGATGCAACTTC280.12498326117037896No Hit
ATTGATACCATATATCCTACAAACTTGACCAAAAGCTTGCTTCCATATCC260.11605588537249477No Hit
ATATTAAATAAGTTAAAATTAATAAATGAAAATAATTGAAAAGTTAAATC250.11159219747355265No Hit
AATGCTAATGATTTTCATCGACCATTCATCGTCCTGGTCGACGAATCCTC250.11159219747355265No Hit
AAATCTATCGGGTTCGAGGAATGGTCGACCAGGGGTTGAAATCGTCGACC250.11159219747355265No Hit
ATCATCTCATAAATGTTTCTATTGTAGAAATTGGAGCATTTTTTCGTGAC240.10712850957461054No Hit
CCCAGTAATAACCAGTTTATGTTCGAGTATTCTCCAAGATCACCAAACAT230.10266482167566844No Hit
CGAGACTTCATCGACCGGGTCCGAGGAATCGTCGACCAGGACGATGAATG230.10266482167566844No Hit
GAGTTTGTTATGGCCTAACTCGAGGAACCTAAGAATCGGTGCGAGTGATG230.10266482167566844No Hit
TGTAAATCATGGTTTTAACTAAATTGGATAAATTAATAAATCTTTTAAAT230.10266482167566844No Hit
GCATCAAACTTAAACTTCCTTCTTGCATTCGTGCTCCTCCGGAAGAACAC230.10266482167566844No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGA207.760407E-570.0000124
ACAGCGG207.760407E-570.000012
CGCTGAA207.760407E-570.000016
GGTTGCA207.760407E-570.0000167
ACAGAGT207.760407E-570.0000126
TACAGAG207.760407E-570.0000125
GGACTTC252.7510578E-670.000017
GGACTTA207.760407E-570.000017
TAACGGA207.760407E-570.000013
AGGCGGA207.760407E-570.000013
AGGCGCT207.760407E-570.000013
GAATGCT207.760407E-570.0000136
CTGAATG207.760407E-570.000018
TCACGGA207.760407E-570.000013
GCGCTGA207.760407E-570.000015
TTGGTTG207.760407E-570.0000125
CTCGGAC252.7510578E-670.000014
CGCGGAC550.070.000014
GAATAGA207.760407E-570.0000144
GAATAAG207.760407E-570.0000149
Produced by FastQC (version 0.11.5)