FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_ColTGA5_Col_UB_i2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_ColTGA5_Col_UB_i2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30982797
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1945650.6279775192665789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGT78250.016.81770727
AGGCCGG21650.016.81277737
TAGGGGG87400.016.4581550
GGGGAGA202750.014.69067722
GGGGGTA177300.013.97584265
GGGGTAA147650.013.46379966
GCCGGGG76050.013.06997439
GGCCGGG79200.012.59435738
CCGGGGG90750.012.57260240
TGGGGGA96200.012.33364420
CCGGGTG20800.012.11499940
AGGCGGG67950.012.05286837
AGGGCGG35150.011.74953537
AGGGGGG415650.011.6535751
ACGGGGG39950.011.65169440
GGGAGAG161250.011.63424523
TGTGGGG143850.011.60584433
GTAGGGG66200.011.2610849
GGGGTTA126300.011.0843366
AAGGGGG143550.010.65439951
Produced by FastQC (version 0.11.5)