FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_ColTGA5_Col_B_i3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_ColTGA5_Col_B_i3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3844684
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108060.2810634111932216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACT226100.059.57245
TAAACTG86850.058.0314876
TAAACTA164550.057.7913446
TAAACTC122900.057.2135736
ACTGTAG44550.056.564589
CTGTAGA44950.054.50396310
TTAAACT135350.054.4124225
GTAAACT71650.053.787545
CTAAACT80600.052.5485735
TAAACTT140400.052.2766
AACTGTA52400.049.4265378
AATAAAC120550.048.519894
AAACTGT64300.046.647637
GTAGAAG54900.043.79640612
CATAAAC71500.043.325924
AAACTAG50050.043.2159547
CGTAAAC26800.042.8400154
AAACTAA126600.041.4960487
TATAAAC77650.041.1115574
TGTAGAA59250.040.69964211
Produced by FastQC (version 0.11.5)