FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_ColTGA5_Col_B_i2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_ColTGA5_Col_B_i2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4334406
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105240.24280143576766922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACT210550.058.6168445
TAAACTA157550.057.536936
TAAACTC112250.057.4651156
TAAACTG88950.056.7393886
CTGTAGA44700.056.06099710
ACTGTAG48250.053.8222549
TTAAACT130550.053.515435
CTAAACT75750.052.722795
GTAAACT68700.051.1021965
TAAACTT127400.049.202996
GCGCTAT7800.047.5670975
AATAAAC121000.046.458544
AACTGTA57350.046.258458
AAACTGT68750.046.0204667
AGCGCTA6100.044.7579464
GCGCTCC2650.043.587655
CAGCGCT4200.043.340563
GTAGAAG57200.043.01397712
AAACTAG50150.042.6408237
TGTAGAA58350.042.58658611
Produced by FastQC (version 0.11.5)