FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_ColTCP21_Col_UB_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_ColTCP21_Col_UB_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22042739
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1700810.7715964880770942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG86700.021.55618950
GGGGAGA166150.018.8749622
TTGGGGG273600.018.28002535
TGGGGGA78700.017.34452220
GGGGTAA124900.015.69207366
GGGGTTA111400.015.17458165
GGGAGAG123200.015.02876723
GGGGGTA159050.014.80935465
GTAGGGG60900.014.76976249
GTTGGGG197400.014.7517934
GGGGAAT103900.014.0803263
CGGGGGT41250.013.82981739
GGGTTAA113950.013.51428666
GGTTGGG179950.013.08976133
TAAGGGG48550.013.04785550
AGGGGGG313850.012.90214851
GGGTGGT176550.012.82632242
GGGGGAT140800.012.82622363
GAGGGTG70800.012.40800927
GGGTAAA158850.012.36036667
Produced by FastQC (version 0.11.5)