FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_ColTCP21_C24_UB_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_ColTCP21_C24_UB_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18561834
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG389120.20963445745716724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTA28850.09.34102365
GGGGGGA81150.09.31598620
TAGGGGG23100.07.878431350
GGGGGAG60850.07.534845421
GGGGGAA56300.06.589630621
AGGGGGG46850.06.27506351
GGGGTAA38900.06.207978766
GGGGTTA40000.05.51226565
GGGGGAT35600.05.407058263
GGGGGTT67350.05.24846364
GGGGTTT88900.05.118016729
Produced by FastQC (version 0.11.5)