FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_ColSPL9_F1b_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_ColSPL9_F1b_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5788032
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102800.17760786395099407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGGCC6750.00226047366.22207664
CAGTCCG7150.00430160955.87398761
TCGAGGG7600.0084521665.52618558
CGAGGGT9200.00213306235.3259619
GTTCCGC10050.00113646225.223757714
CCGTCCC10250.00147665895.12191970
GTCGTGT30850.05.10522866
Produced by FastQC (version 0.11.5)