FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_C24SPL9_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_C24SPL9_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7898163
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168970.21393582279828866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCC13850.07.83369970
TCCGGGC7351.5622598E-57.142958647
GTCCCCC5600.00221362526.87479368
GCGTGCG9106.477545E-66.538554741
CGTCCCC6450.00135467676.51143267
TAGGGGG9104.527076E-56.15362250
ACACGGG12007.7307246E-55.24980953
GGGGGGA29500.05.22021520
Produced by FastQC (version 0.11.5)