FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_AtSG91_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_AtSG91_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023356
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53530.2645604629140893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACT28900.032.2164545
TAAACTG13100.027.5193676
ACTGTAG6450.026.0461359
TAAACTA24900.025.582596
TAAACTC18050.025.0141096
TTAAACT24600.020.0621725
TAAACTT24950.019.9200446
CTAAACT13600.019.3026245
CTGTAGA8100.019.01207210
GTAAACT13700.018.9062375
AACTGTA10700.018.644598
AATAAAC25150.017.9538024
GTGTGCC9450.016.66601459
AGTGTGC10550.014.92832558
GCCTCTA9900.013.78733963
GTGCCTC10250.013.65800161
CATAAAC16850.013.5026234
TGTGCCT11200.013.43697460
AAACTGT15950.013.38538557
CTATGTG10550.013.26962267
Produced by FastQC (version 0.11.5)