FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_AtSG141_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_AtSG141_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392700
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61180.2556944038115936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG2650.001871666410.56565450
GGGGGAG11102.0008883E-117.882925521
GGGGAGA8553.7787686E-87.77781922
GGGGGGA14150.07.667886320
TGGGGGG17100.07.368152635
GATCCTA9805.4507836E-87.14394044
GGGGTGT5450.00166087077.06396341
TCCTAAC6107.17861E-46.88557056
GTGGGGG14703.8198777E-116.66642434
ATCCTAT12752.3954271E-86.31441935
TGGTGGG8903.268588E-56.29190632
TTGGGGG10205.711323E-66.17624535
GGGAGAG8255.3430634E-45.939425523
AGGGGGG8609.0863934E-45.697467351
GGTGGGG16104.756821E-75.21720133
Produced by FastQC (version 0.11.5)