FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_AtSG138_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_AtSG138_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333579
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59150.2534733128812009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGGG2408.1775815E-411.66626635
GCCGTTA2750.00254676610.18125150
TGTGGGG6201.2686502E-57.90312533
GGGGGGA15100.07.648908620
AGGGGGG9959.276846E-97.386523251
CGGGGGG10752.9488365E-76.511683541
GGGGAGA8257.789481E-56.36382722
GGGGGAG10551.4739071E-66.30323621
GAGGGGG7950.00221772775.72295250
GCGGGGG10150.00129581385.17212639
TAGGTAT9600.0036233515.1047584
Produced by FastQC (version 0.11.5)