FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_AtSG137_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_AtSG137_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161307
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51230.2370324993163859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACT22900.013.4520365
ACTGTAG5001.8189894E-1213.2994599
CGGACTG2701.6735926E-411.6664636
TAAACTA19500.011.1280116
TAAACTC15150.010.6268776
TAAACTG10750.010.4184226
CTAAACT11950.08.2022115
AGGGTGT6151.1388534E-57.96715668
GATAGGG5855.269308E-57.777461565
CTGTAGA7957.744711E-77.483972510
TTAAACT21350.07.3782865
ATAGGGT7151.059014E-57.34235966
GGGTGTA5250.00111725787.33303569
GTAAACT10051.1197699E-87.3146585
GTAGAAG10856.531991E-97.096485612
TGTGGGG5450.00166035287.064096548
GGGGTTA5000.0056361196.999715365
GGATAGG5500.00182906486.99971564
TAAACTT21100.06.9667026
AGGGGGG6602.3391368E-46.89365951
Produced by FastQC (version 0.11.5)