FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n02_AtMCY2_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n02_AtMCY2_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518690
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75630.30027514303070246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTA4954.3928594E-911.31307365
GGGGGGA14350.09.51252420
TGTACCG3000.00522160679.3343975
GGGGGAA9101.6370905E-98.07704321
GGGGGAG11301.4710167E-86.8142621
GGGGTAA6259.4701885E-46.71996666
GGGGAGA7601.853764E-46.447463522
TGGGGGT9000.0095469975.055429539
Produced by FastQC (version 0.11.5)