FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24601643
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG415800.16901310209240902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG19150.033.89420342
CCGCTCA32600.030.23606133
CGCTCAT33200.029.71084234
GTCACTC40000.029.06460629
CAGTCAC92700.029.01734227
CCAGTCA95100.028.9101826
AATCGGG26450.028.90865941
CTCCGGA35750.028.64740633
CTCCAGT103550.027.95507424
TCCGGAG37200.027.9265634
AGTCACC43100.027.36000628
AGTCACT46700.026.59560428
CACCGCT37300.026.59404631
GAACTCC115050.026.42015621
GCGCTAG3350.026.0041475
GTCACCG41050.025.93981429
CGTCTGA120050.025.93694516
CGCTAGA6400.025.5897066
ACGTCTG122550.025.5205815
CTGAACT122200.025.4523119
Produced by FastQC (version 0.11.5)