FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_ColTGA5_Col_UB_i3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_ColTGA5_Col_UB_i3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22403
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG820.36602240771325273TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGT430.19193857965451055TruSeq Adapter, Index 6 (97% over 36bp)
CGCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGTG300.1339106369682632TruSeq Adapter, Index 6 (96% over 33bp)
ATCTTGAGTACCGTCGTTAAATCCACGTCAGCCAACCATGGATGAGCCAC280.12498326117037896No Hit
GGTGATAATATAAAGTTTCATTAATCCGAGATATTTAGTGTCTTCGGTTT280.12498326117037896No Hit
ATCATCTTCGACATTAATGAAGGCAGATCAACCAGTTATAAACTTAATTG260.11605588537249477No Hit
CCAGGCGCTAAAAATCTTAAGTTGAAGGAGAGGTTTGAAGGTGGGGATTC250.11159219747355265No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT240.10712850957461054TruSeq Adapter, Index 6 (97% over 36bp)
GGTATTAATGAAATCGTCGACCAGGTCCGAGACTTCATCGACCGGGTCCG240.10712850957461054No Hit
GAACCTTATAAAAACCGTATTGACTCTGCGCAAAAAACTACAGGATTGAC240.10712850957461054No Hit
GACCATTCATCGTCCTGGTCGACGATTCCTCGGACCCGGTCGATGAATTC230.10266482167566844No Hit
AGAGGCTTAAAAGTCTAGGTGCTGATATTGCCGAAAATGAGACTTAGGAC230.10266482167566844No Hit
AGACTTTTCTTTTTTATCCAATAGACTTACATATAACTTTTGGCTTAAGA230.10266482167566844No Hit
CTATATCGAATTGGACCGCCAAGCTCGGCTTCATCTGGTAAGTGTATAGG230.10266482167566844No Hit
ATCAATAATCTTCTTTCAAACCCCCGATTAATTTATTTTGGCGTTTGTAA230.10266482167566844No Hit
TCAATGTTTTGTTTATTTTTTCTCCAAACTTGTGTAAACTTCGTTGTGTT230.10266482167566844No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAGG207.760407E-570.0000127
ACTGTCT252.7510578E-670.000019
ACACGGA252.7510578E-670.000013
AAACCGA207.760407E-570.0000153
TAAACGG252.7510578E-670.000012
AAATCGG207.760407E-570.000012
GGCGCTA207.760407E-570.000014
GGACTCT900.070.000017
TACGGAC550.070.000014
GGATTCA207.760407E-570.0000145
CGGACTC1000.070.000016
CGAAGCA207.760407E-570.0000157
GGTTTGA207.760407E-570.0000132
AGGCGCT207.760407E-570.000013
ACTTCTT207.760407E-570.000019
CGCTAAA207.760407E-570.000016
AGGTGGG207.760407E-570.0000139
TGGGGAT207.760407E-570.0000142
TCTGTAG207.760407E-570.0000111
CCCCGGA207.760407E-570.000013
Produced by FastQC (version 0.11.5)