FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_ColTGA5_Col_UB_i2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_ColTGA5_Col_UB_i2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30982797
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT1973840.6370761167882938TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT1536410.49589131672004955TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG485380.15666113036857193TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT460930.1487696543343069TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG568050.060.47374742
CGGGGGT251350.057.6758944
AATCGGG678500.057.40244741
TCGGGGG329350.055.90797443
GAATCGG716750.055.07157540
GCGGGGT186050.053.76485452
AGAATCG816700.052.9258139
CGGGGTG182900.052.39449353
TCCGGAG912950.052.379634
GCGGCGT89750.052.17801752
ACTCCGG929500.051.48470732
CCGGAGA927800.051.44315735
CGGAGAA932850.051.037136
CTCCGGA939200.050.9939733
TCGGGGT233550.050.87718243
CACTCCG955600.050.1334631
CGGGGTT225350.049.87074744
GGAGAAT960050.049.4343737
GAGAATC953400.048.13470538
TGCGGGG95500.048.12018651
Produced by FastQC (version 0.11.5)