FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_ColTGA5_Col_B_i3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_ColTGA5_Col_B_i3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3844684
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTAG59700.059.9739849
ATAAACT231150.059.720245
TAAACTG107950.059.5296
CTGTAGA57000.059.3149110
TAAACTA168350.058.0055586
TAAACTC111050.056.0391736
GTAAACT82400.054.6675835
AACTGTA68450.054.4549988
TTAAACT134700.054.4370965
AAACTGT82850.051.9606067
TAAACTT139450.051.5035746
CTAAACT77850.050.804055
GTAGAAG63800.050.4694712
AATAAAC122950.049.6189274
TGTAGAA68450.048.2169611
AAACTAG54400.042.9129647
CGTAAAC26250.042.267694
GATAAAC59550.042.08334
ATTAAAC79400.041.6571774
AAACTAA132000.040.77977
Produced by FastQC (version 0.11.5)