FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_ColTGA5_Col_B_i2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_ColTGA5_Col_B_i2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4334406
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT68820.15877608142845873TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACTG102200.059.6256076
ATAAACT214650.058.8655555
ACTGTAG60350.058.4005749
CTGTAGA56650.057.76657510
TAAACTA155700.057.683686
GGGCGCT4750.055.267223
TAAACTC104600.055.1120876
AACTGTA68250.053.3842288
AGCGCTC5900.052.7986644
GTAAACT81200.052.760675
TTAAACT121500.052.7180985
AAACTGT81800.052.3724677
CTAAACT71600.052.2087365
GCGCTAG7450.052.149685
AGCGCTA6250.052.0820274
CGCTAGA21500.049.4902956
GCGCTAT6600.049.32015
GAGCGCT8350.049.0455253
GGCGCTA8600.048.8391044
TAAACTT127650.048.7523886
Produced by FastQC (version 0.11.5)