Basic Statistics
Measure | Value |
---|---|
Filename | HL52FAFXY_n01_ColTCP21_Col_UB_i.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22042739 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 38 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT | 633468 | 2.8738170877947606 | TruSeq Adapter, Index 2 (97% over 37bp) |
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC | 56967 | 0.2584388446463028 | TruSeq Adapter, Index 2 (97% over 34bp) |
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 26141 | 0.11859234008985908 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 77170 | 0.0 | 61.322784 | 48 |
GCCGTCT | 76695 | 0.0 | 61.223972 | 51 |
TGCCGTC | 77180 | 0.0 | 61.19268 | 50 |
ATGCCGT | 77420 | 0.0 | 61.079556 | 49 |
CGTATGC | 77895 | 0.0 | 60.8374 | 46 |
GTATGCC | 78140 | 0.0 | 60.669044 | 47 |
CTCGTAT | 78560 | 0.0 | 59.32906 | 44 |
TCGTATG | 79900 | 0.0 | 59.15306 | 45 |
CCGTCTT | 79900 | 0.0 | 58.82068 | 52 |
CTGCTTG | 79965 | 0.0 | 58.217533 | 59 |
TCTCGTA | 80740 | 0.0 | 57.68815 | 43 |
TATCTCG | 81805 | 0.0 | 56.98846 | 41 |
CGTCTTC | 83025 | 0.0 | 56.57299 | 53 |
ATCTCGT | 83035 | 0.0 | 56.131645 | 42 |
CTTCTGC | 83570 | 0.0 | 56.05328 | 56 |
CGCTCAT | 87100 | 0.0 | 54.58911 | 34 |
GCTTGAA | 86405 | 0.0 | 54.38881 | 61 |
TGCTTGA | 86035 | 0.0 | 54.289127 | 60 |
TTCTGCT | 86110 | 0.0 | 53.98935 | 57 |
TCTGCTT | 87365 | 0.0 | 53.42187 | 58 |