FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_ColTCP21_Col_UB_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_ColTCP21_Col_UB_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22042739
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT6334682.8738170877947606TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC569670.2584388446463028TruSeq Adapter, Index 2 (97% over 34bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG261410.11859234008985908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG771700.061.32278448
GCCGTCT766950.061.22397251
TGCCGTC771800.061.1926850
ATGCCGT774200.061.07955649
CGTATGC778950.060.837446
GTATGCC781400.060.66904447
CTCGTAT785600.059.3290644
TCGTATG799000.059.1530645
CCGTCTT799000.058.8206852
CTGCTTG799650.058.21753359
TCTCGTA807400.057.6881543
TATCTCG818050.056.9884641
CGTCTTC830250.056.5729953
ATCTCGT830350.056.13164542
CTTCTGC835700.056.0532856
CGCTCAT871000.054.5891134
GCTTGAA864050.054.3888161
TGCTTGA860350.054.28912760
TTCTGCT861100.053.9893557
TCTGCTT873650.053.4218758