FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_ColTCP21_C24_UB_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_ColTCP21_C24_UB_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18561834
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT574230.3093605944326407TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG91150.047.22901548
GCCGTCT91900.046.38756651
CGTATGC95050.045.21751846
ATGCCGT95800.044.7904549
TGCCGTC95850.044.65838250
GTATGCC97450.044.2475647
TCGTATG116550.037.0263845
CCGTCTT116650.036.93542552
CTCGTAT116400.036.89398644
CTGCTTG128450.033.35172359
TATCTCG131200.032.8121941
CGCTCAT132600.032.78232234
TCTCGTA131950.032.7317943
CCGCTCA137400.031.56075333
ATCTCGT143950.030.0518242
ACCGCTC146200.029.804732
CGTCTTC146000.029.6542353
CACCGCT154200.028.30380831
CTTCTGC158050.027.41548556
TCACCGC163700.026.532930
Produced by FastQC (version 0.11.5)