FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_ColSPL9_F1b_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_ColSPL9_F1b_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5788032
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7650.026.0780548
TGCCGTC8000.024.06235550
ATGCCGT10500.018.99972349
GCCGTCT10550.018.91000451
GTATGCC11400.017.19273247
CGTATGC14850.013.19846146
GTCACGA15050.012.79062229
AGTCACG15650.012.74752828
TCGTATG21000.010.66651245
CATCTCG17300.010.52007941
TCACGAG19400.010.28344430
CTCGTAT20050.010.12454144
CCGTCTT21700.010.00002852
CACGAGA23550.08.91714531
CCAGTCA23100.08.78782726
CAGTCAC23800.08.38230327
CGTCTTC28350.08.27162653
CGCTGGG3900.0048587618.07687565
ATCTCGT26500.08.05648642
CTGCTTG25200.08.05557759
Produced by FastQC (version 0.11.5)