FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_ColSPL9_F1a_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_ColSPL9_F1a_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6575251
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT84600.1286642897738809TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15650.039.58450750
TATGCCG15950.039.0591148
GCCGTCT16850.037.18086251
ATGCCGT18750.033.5996149
GTATGCC19300.032.82345247
CGTATGC23300.027.3387446
GTCACGA24950.025.95160129
AGTCACG26900.023.68002128
CTCGTAT29450.021.86748544
CATCTCG27050.021.73743441
TCACGAG30300.021.48489830
TCGTATG31750.020.61393445
CCAGTCA31550.020.30087526
CAGTCAC31750.020.28322827
CCGTCTT33200.019.39751252
TCCAGTC33950.019.27812625
CACGAGA34950.018.5262531
CTGCTTG35600.017.5985159
ATCTCGT35250.017.47510742
CGTCTTC39600.017.05801253
Produced by FastQC (version 0.11.5)